منابع مشابه
Study of sex differences in the expression of β-actin gene in various tissues of the rat
Introduction: Beta-actin is a housekeeping gene, which is used as an internal standard in various biological studies. Recently, it was reported that the expression of commonly used housekeeping genes including ß–actin changes in disease states or under certain experimental conditions. Based on the reports about the effects of sex hormones on the expression of the ß- actin gene, in this study...
متن کاملCloning and sequence analysis of the coding sequence of β-actin cDNA from the Chinese alligator and suitable internal reference primers from the β-actin gene.
β-Actin is an essential component of the cytoskeleton and is stably expressed in various tissues of animals, thus, it is commonly used as an internal reference for gene expression studies. In this study, a 1731-bp fragment of β-actin cDNA from Alligator sinensis was obtained using the homology cloning technique. Sequence analysis showed that this fragment contained the complete coding sequence ...
متن کاملMicroRNA-mediated up-regulation of an alternatively polyadenylated variant of the mouse cytoplasmic β-actin gene
Actin is a major cytoskeletal protein in eukaryotes. Recent studies suggest more diverse functional roles for this protein. Actin mRNA is known to be localized to neuronal synapses and undergoes rapid deadenylation during early developmental stages. However, its 3'-untranslated region (UTR) is not characterized and there are no experimentally determined polyadenylation (polyA) sites in actin mR...
متن کاملCloning and sequence analysis of an actin gene in aloe.
Aloe (Aloe spp), containing abundant polysaccharides and numerous bioactive ingredients, has remarkable medical, ornamental, calleidic, and edible values. In the present study, the total RNA was extracted from aloe leaf tissue. The isolated high-quality RNA was further used to clone actin gene by using reverse transcription-polymerase chain reaction (RT-PCR). The result of sequence analysis for...
متن کاملCloning and Molecular Characterization of Porcine β-casein Gene (CNS2)
The production of therapeutic proteins from transgenic animals is one of the most important successes of animal biotechnology. Milk is presently the most mature system for production of therapeutic proteins from a transgenic animal. Specifically, β-casein is a major component of cow, goat and sheep milk, and its promoter has been used to regulate the expression of transgenic genes in the mammar...
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ژورنال
عنوان ژورنال: Anatomical Science
سال: 2018
ISSN: 2578-1804
DOI: 10.24294/as.v1i1.298